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rabbit polyclonal anti na v 1 5  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit polyclonal anti na v 1 5
    Rabbit Polyclonal Anti Na V 1 5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+na+v+1+5/Anti-Nav1%2E5+(SCN5A)+(1978-2016)+Antibody/pmc11288740-104-35-41
    Average 93 stars, based on 27 article reviews
    rabbit polyclonal anti na v 1 5 - by Bioz Stars, 2026-10
    93/100 stars

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    Membrane:

    Article Title: Detrimental proarrhythmogenic interaction of Ca 2+ /calmodulin-dependent protein kinase II and Na V 1.8 in heart failure
    Article Snippet: Protein concentration was determined by BCA assay (Pierce Biotechnology). .. Denatured tissue homogenates (10 min, 70 °C in 2% beta-mercaptoethanol) were separated on 8% SDS-polyacrylamide gels, then transferred to a nitrocellulose membrane, and incubated with the following primary antibodies: rabbit polyclonal anti-CaMKIIδ (1:5000, Thermo Scientific, PA5-22168), rabbit polyclonal anti-Na V 1.5 (1:2000, Alomone labs, ASC-005), and mouse monoclonal anti-GAPDH (1:20000, BIOTREND, BTMC-A473-9) at 4 °C overnight. .. Secondary antibodies included HRP-conjugated goat anti-rabbit and goat anti-mouse (1:20000, Jackson Immunoresearch, 111-035-144 and 115-035-062, respectively).

    Incubation:

    Article Title: Detrimental proarrhythmogenic interaction of Ca 2+ /calmodulin-dependent protein kinase II and Na V 1.8 in heart failure
    Article Snippet: Protein concentration was determined by BCA assay (Pierce Biotechnology). .. Denatured tissue homogenates (10 min, 70 °C in 2% beta-mercaptoethanol) were separated on 8% SDS-polyacrylamide gels, then transferred to a nitrocellulose membrane, and incubated with the following primary antibodies: rabbit polyclonal anti-CaMKIIδ (1:5000, Thermo Scientific, PA5-22168), rabbit polyclonal anti-Na V 1.5 (1:2000, Alomone labs, ASC-005), and mouse monoclonal anti-GAPDH (1:20000, BIOTREND, BTMC-A473-9) at 4 °C overnight. .. Secondary antibodies included HRP-conjugated goat anti-rabbit and goat anti-mouse (1:20000, Jackson Immunoresearch, 111-035-144 and 115-035-062, respectively).

    Article Title: Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing
    Article Snippet: Peptides for GFP splicing were sourced from Proteogenix, France. .. Membranes were incubated with rabbit polyclonal anti-Na V 1.5 (#ASC-005, Alomone labs; 1:2000), anti-Na V 1.5 (#ASC-013, Alomone labs; 1:1500) or anti-P2X2 Antibody (#APR-003, Alomone labs; 1:2000) and the bound primary antibodies were detected by a HRP-conjugated goat anti-rabbit secondary antibody (W401B, Promega; 1:2000). .. Membranes were developed and visualized using the PierceTM ECL immunoblotting substrate (Thermo Fisher Scientific).

    Article Title: Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing
    Article Snippet: .. Membranes were incubated with rabbit polyclonal anti-Na V 1.5 (#ASC-005, Alomone labs; 1:2000), anti-Na V 1.5 (#ASC-013, Alomone labs; 1:1500) or anti-P2X2 Antibody (#APR-003, Alomone labs; 1:2000) and the bound primary antibodies were detected by a HRP-conjugated goat anti-rabbit secondary antibody (W401B, Promega; 1:2000). .. Membranes were developed and visualized using the PierceTM ECL immunoblotting substrate (ThermoFisher Scientific).

    Article Title: Empagliflozin inhibits increased Na influx in atrial cardiomyocytes of patients with HFpEF
    Article Snippet: After denaturation (30 min at 37°C for Na V 1.5 or 5 min at 95°C for CaMKII and GAPDH at 500 rpm in 2% β-mercaptoethanol (Sigma Aldrich)), proteins were separated on 8% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane (GE Healthcare). .. Membranes were incubated with primary antibodies (rabbit polyclonal anti-phospho-Na V 1.5 (at serine 571, 1:1000, a kind gift from Prof. Dr. P. Mohler and Prof. Dr. T. Hund, The Ohio State University, Columbus, Ohio, USA), rabbit polyclonal anti-Na V 1.5 (1:200, Alomone Labs, catalog number ASC-013), mouse monoclonal anti-CaMKII (1:1000, BD Biosciences, catalog number 611293), mouse monoclonal anti-phospho-CaMKII (1:1000, Affinity BioReagents, catalog number MA1-047), or mouse monoclonal anti-GAPDH (1:20000, Abcam, catalog number G8795)) overnight at 4°C. .. Secondary antibodies HRP-conjugated sheep anti-mouse IgG (1:3000 for anti-CaMKII, 1:5000 for anti-phospho-CaMKII, 1:30 000 for anti-GAPDH, GE Healthcare, catalog number NA931VS) and donkey anti-rabbit IgG (1:5000 for anti-phospho-Na V 1.5 and for anti-Na V 1.5, GE Healthcare, catalog number NV934 V) were incubated for 1 h at room temperature.



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